Effects of Heavy Metal Ions on Living Tissues

Heavy metal EPR spectroscopy is a long known application throughout all miniscope series it is a modern application for the MS5000 with best signal to noise ratio for standard and research applications. Applications reaching from biochemistry, medical application throughout chemical characterisation.

Examples for heavy metal EPR spectroscopy by Miniscope:

Heavy metals are rather efficient in catalysing chemical reaction with radicals at least as intermediates. This may in part explain their toxicity. Plant roots e.g. produce different DMPO adducts if there have been exposed to cadmium (1 µM,    1 week) or simply were grown in normal aqueous culture solution. Fig. 22 shows the result of the experiment, Fig. 23 that of the control.

While the control spectrum is dominated by a three line signal derived from a double substituted DMPO molecule the cadmium exposure resulted in an increase of the part of the monosubstituted adduct with six lines.

After incubation with DMPO the culturing medium (Fig. 24) shows an ESR spectrum different from those obtained from the roots, the three line component of the double substituted compound is replaced by another one typical for the hydroxyl adduct. Radicals must react differently in the plant tissue and in free solution [9].

Miniscope ESR-spectrum of DMPO-adducts from plant roots exposed to cadmium, overlaid with a fitted simulation (MiniScope MS100: Scan time 60 s, scan 10 mT, 9.4 GHz).
Miniscope ESR-spectrum of DMPO-adducts from plant roots exposed to cadmium, overlaid with a fitted simulation (MiniScope MS100: Scan time 60 s, scan 10 mT, 9.4 GHz).
Miniscope ESR-spectrum of culturing medium for plant roots, DMPO added (50 mM), together with fitted simulation (parameter settings see Fig. 22).