ESR applications in Life Sciences

In 21st century Life Science is driven by the desire to elucidate fundamental life processes. Very sensitive and easy to use benchtop spectrometers enable scientists to measure reactive oxygen species (e.g. nitric oxide), refine knowledge on biomolecule's structure and function and monitor the path of oxygen throughout living organisms.

The list of applications seems to be endless with our state-of-the-art EPR spectrometer MS5000.

EPR/ESR spectroscopy in life sciences

The list of applications seems to be endless with our state-of-the-art EPR/ESR MS5000 spectrometer. Some examples are spin trapping applications as for nitric oxide (NO), protein structure, fluoride or silicate investigations.

Spin labelling involves extrinsic probes as nitroxide attached to the protein or molecule under investigation. 

Advantages of ESR/EPR spectrometer MS5000

  • High sensitivity and spectrometer stability also for aqueous samples due to powerful automatic spectrometer self-adjustment routines

  • Auto-sampler for efficient routine investigations

  • Measurement and user interface parameter setting by recipes for extremely easy handling of routine measurements, also by unskilled persons 

  • Automatic time- dependent and transient EPR- measurements in timescales from 2µs time- resolution to hours or days

  • g- value representation of EPR spectra

  • Very high reproducibility of EPR line positions by automatic microwave-frequency readjusted magnetic field scale

  • X- ray irradiation facility (accessory, option)


Reactive oxygen species (ROS)

Spin trapping of nitric oxide (NO) with iron complexes, determination of oxidative stress, in vivo application of ESR

Detection and Quantification of Oxygen Radicals in Whole Blood

Spin trapping technique in combination with ESR  MS5000 measurements are standard applications for oxymetry in blood.

Protein structure and enzyme function

structural elucidation of biomolecules, specific analysis of active centers and mechanisms of enzymes